Natural killer (NK) cells attack tumor and infected cells, but the receptors and ligands that stimulate them are poorly understood.
Two murine ligands for the lectin-like receptor NKG2D, H60 and retinoic acid early inducible (Rae-1a B, g, d and e), are distant relatives of major histocompatibility complex class I molecules.
These molecules are encoded by Rae-1 and H60 minor histocompatibility antigen genes on mouse chromosome 10 and show weak homology with MHC class I.
Expression of the NKG2D ligands is low or absent on normal, adult tissues; however, they are constitutively expressed on some tumors and upregulated by retinoic acid.
Ectopic expression of Rae-1 and H60 confers target susceptibility to NK cell attack.
NKG2D binds to H60 with approximately 25-fold higher affinity than to Rae-1.
Rae-1 and H60 compete directly for occupancy of NKG2D; therefore, NKG2D can be occupied by only one ligand at a time.
Additionally, Rae-1 and H60 ligands of the NKG2D receptor stimulate tumor immunity.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human RAE1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human RAE1.
