The DNA repair proteins Rad50, Rad51A-D, Rad52 and Rad54 A and B are key components of the double-strand break repair pathway (DSBR).
The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair.
Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination.
In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3.
Rad51B is a widely expressed protein that is involved in the repair of double-stranded DNA breaks that can arise during DNA replication.
It can form a dimer with Rad51C and this complex shows single-stranded DNA dependent ATPase activity.
Applications:Suitable for use in Western Blot, Immunohistochemistry, and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Rad51B.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Rad51B.
