QPCT (glutaminyl-peptide cyclotransferase) is a 361aa protein that belongs to the glutaminyl-peptide cyclotransferase family.
QPCT is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.
QPCT binds one zinc ion per subunit and has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue.
The human QPCT gene shares 86% overall sequence identity with the bovine homolog.
QPCT contains an N-terminal signal peptide region, several glycosylation and phosphorylation sites and two cysteine residues conserved between the bovine and human enzymes.
Existing as two alternatively spliced isoforms, the QPCT gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, fruit fly, mosquito, M.grisea and N.crassa, and maps to human chromosome 2p22.2.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human QPCT.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human QPCT.
