Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-myc.
The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-myc expression.
The FBP interacting repressor, FIR, binds to the central DNA-binding domain of FBP and can serve as an overriding negative regulator of c-myc promoter activity.
FIR interacts with the TFIIH complex, which is a multifunctional, multisubunit RNA polymerase II transcription factor that interacts with several DNA-binding transactivators.
FIR blocks activator-dependent, but not basal transcription through TFIIH.
FIR shares identity with seven in absentia (siah) binding protein 1.
FIR is expressed in spleen, thymus, prostate, small intestine, colon, and peripheral blood leukocytes, and with relatively higher levels of expression in testis and ovary.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PUF60. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human PUF60.
