In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S Proteasome.
The 26S Proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains.
It is made up of two multisubunit complexes: the 20S Proteasome chamber, which serves as the proteolytic core of the complex and two 19S regulatory particles which recognize and unfold ubiquitinated proteins.
PSMD12 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 12), also known as p55 or Rpn5, is a 456aa protein belonging to the proteasome subunit p55 family.
PSMD12 acts as a regulatory subunit of the 26S proteasome and is a component of the PA700 complex.
PSMD12 contains one PCI domain.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PSMD12. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human PSMD12.
