Prostate stem cell antigen (PSCA) is a 123aa glycosylated protein that shares homology with the Thy-1/Ly-6 family of glycosyl-phosphatidylinositol GPI)-anchored cell surface antigens.
The PSCA gene maps to chromosome 8q24.2 and transcripts are most prevalent in prostate and placenta.
The gene encoding c-myc is also located on chromosome 8q and like PSCA, is overexpressed in a large number of prostate cancers.
Transcripts for PSCA are also abundant in urothelial tumors, and levels of PSCA transcripts increase in confluent RT112 bladder carcinomas, suggesting that PSCA is a marker for urothelial and gastric tissue carcinogenesis.
Among prostate cancer cell surface antigens, PSCA is expressed in over 80% of prostate carcinomas and correlates well to certain prostate cancer phenotypes such as prostate cancer bone metastates.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PSCA. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human PSCA.
