Protamines are small, arginine-rich (basic), nuclear proteins that mediate normal sperm head condensation and DNA stabilization.
Mice, humans, and certain fish have 2 or more different protamines, whereas the sperm of bull, boar, rat, rabbit, guinea pig, and ram have one form of protamine.
The majority of DNA in human sperm is bound to protamines with only a small proportion of DNA bound to histones in a way similar to active chromatin.
The retention of histone association with sperm DNA with respect to protamine association to sperm DNA can change within as little as 400 bp of DNA, suggesting that there is fine control over haploid DNA organization.
Protamines eventually replace histones late in the haploid phase of spermatogenesis.
The human protamine 1 gene maps to chromosome 16p13.13 and encodes a 51aa protein.
The human protamine 2 gene maps to chromosome 16p13.13 and encodes a 102aa protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol..
Specificity：Recognizes human PRM2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PRM2.
