In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the serine/threonine protein phosphatases.
In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit.
Protein phosphatase 4 (PP4) is comprised of different regulatory subunits that exhibit mutually exclusive interactions with the PP4 catalytic subunit, PPX.
PP4R1, also known as PPP4R1 or PP4(Rmeg), is regulatory subunit 1 of protein phosphatase 4.
It is ubiquitously expressed and can form a binary complex with PPX that negatively regulates the activity of HDAC3.
PP4 is required for cell growth, nucleation and the stabilization of microtubules during cell division.
This suggests that PP4R1 participates in the regulation of mitosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human PPP4R1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues in human PPP4R1.
