84-4396-45　［取扱停止］PPP1R13B (Apoptosis-stimulating of p53 Protein 1, Protein Phosphatase 1 Regulatory Subunit 13B, ASPP1, KIAA0771) 50ul　361007

特徴

• ASPP proteins interact with p53 and are responsible for enhancing p53-induced apoptosis but not cell cycle arrest.
• Inhibition of endogenous ASPP1 (PPP1R13B) function inhibits the apoptotic function of endogenous p53 in response to apoptotic stimuli.
• ASPP1 amplifies DNA binding and transactivation function of p53 on the promoters of proapoptotic genes in vivo.
• Expression of ASPP1 is often downregulated in human breast carcinomas expressing wildtype p53, but not in those expressing mutant p53.
• This research indicates that ASPP1 regulates the tumor suppression function of p53 in vivo.
• ASPP1 is predominantly a cytoplasmic protein, although some fraction of the polypeptide is nuclear.
• Defects in PPP1R13B, the gene which encodes ASPP1, may be a cause of breast cancers.
• The deduced ASPP1 protein contains 1090aa residues.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.