84-4396-32　［取扱停止］PPAT (Amidophosphoribosyltransferase, Atase, Glutamine Phosphoribosylpyrophosphate Amidotransferase, GPAT) 100ul　361000

特徴

• Atase (amidophosphoribosyltransferase), also known as PPAT (phosphoribosyl pyrophosphate (PRPP) amidotransferase), PRAT or GPAT (glutamine phosphoribosyl pyrophosphate amidotransferase), is a ubiquitously expressed N-terminal nucleophile-type glutamine amidotransferase that belongs to the purine/pyrimidine phosphoribosyltransferase family.
• Existing as a homotetramer, Atase plays an important role in purine metabolism.
• More specifically, Atase functions as regulatory enzyme and contains one glutamine amidotransferase type-2 domain.
• Binding a magnesium ion and a 4Fe-4S cluster as cofactors, Atase catalyzes the first step (the rate-limiting step) in the purine nucleotide biosynthesis pathway, a two-step reaction that results in the formation of phosphoribosylamine from PRPP and glutamine.
• The first step of this reaction is catalyzed by the N-terminal glutaminase domain while the second step is catalyzed by the C-terminal PRTase domain.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.