DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases.
In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches.
These exonucleases include the family of DNA polymerases.
DNA pol g2 (polymerase (DNA directed), gamma 2, accessory subunit), also known as mitochondrial DNA polymerase accessory subunit, POLB, HP55, PEOA4, POLGB, MTPOLB, POLG-BETA or POLG2, is a 485aa subunit of mitochondrial DNA pol g.
DNA pol g2 enhances DNA binding and promotes processive DNA synthesis.
Defects in the gene encoding DNA pol g2 are the cause of PEOA4 (progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human POLG2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human POLG2.
