Endoglycan, also known as podocalyxin-like 2, exists as a disulfide-dependent homodimer and belongs to the CD34 family of proteins.
It is a proteoglycan modified with chondroitin sulfate chains and (with appropriate post-translational modifications) functions as an L-selectin ligand, interacting via sulfation on two tyrosine residues.
Endoglycan contains a membrane proximal globular domain, a single pass transmembrane domain, a mucin-like domain and a highly acidic N-terminal with O-linked sLex structures.
It is widely expressed with distribution including endothelial cells, hematopoietic precursors and leukocyte subpopulations.
Endoglycan is similar to PSGL-1 in that it also exhibits catch-slip transitional bonds.
These bonds help to mediate the rolling and tethering of circulating leukocytes on vascular surfaces, typically during immune surveillance and inflammation.
The ligand activity of Endoglycan is dependent on sialylation and fucosylation and may mediate adhesion events.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human PODXL2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human PODXL2.
