Non-collagenous proteins of the small leucine-rich repeat (SLR) protein family are considered important components of the extracellular matrix.
The extracellular matrix plays an integral role in the pivotal processes of development, tissue repair and metastasis by regulating cell proliferation, differentiation, adhesion and migration.
Podocan, also known as PCAN, SLRR5A or PODN, is a 613aa secreted protein belonging to the small leucine-rich proteoglycan (SLRP) family and the class V subfamily.
Expressed in kidney, heart, liver, pancreas and vascular smooth muscle cells, Podocan exists as three alternatively spliced isoforms containing twenty LRR (leucine-rich repeats), a unique N-terminal cysteine-rich cluster pattern and a highly acidic C-terminal domain.
Podocan is considered a glycoprotein containing N-linked oligosaccharides that may be involved in growth regulation in cardiovascular tissues.
Encoded by a gene located on human chromosome 1, Podocan interacts type I collagen (COL1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human PODN. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human PODN.
