ADPN, a member of the Adiponutrin family, displays lipase activity that is dependent upon the presence of an activated serine residue.
D-glucose elicits a 7-fold increase in ADPN mRNA levels, and insulin has a slight effect on ADPN expression in the presence or absence of glucose.
The glucose-induced increase in ADPN expression can be reversed by factors known to raise intracellular cAMP.
mRNA ADPN levels are negatively correlated with fasting glucose levels and subjects with high ADPN mRNA levels have increased insulin sensitivity, implicating ADPN in obesity and diabetes.
ADPN gene expression in humans is highly regulated by changes in energy balance.
In mice adipocytes, ADPN parallels the expression of fatty acid synthase (FAS) and Srebp1c, a variant of Srebp1.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PNPLA3.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PNPLA3.
