Melanin-concentrating hormone (MCH) is a 19aa cyclic neuropeptide derived from a 165aa pro-MCH precursor.
In addition to the hormone, the pro-MCH precursor contains a 144aa mature MCH as well as a 12aa neuropeptide glycine-glutamic acid (NGE) and a 19aa neuropeptide glutamic acid-isoleucine (NEI).
Mainly expressed in the hypothalamus, the melanin-concentrating hormone modulates feeding behavior, aggression, anxiety, arousal and reproductive function in mammals by controlling the release of luteinizing hormone (LH).
The melanin-concentrating hormone receptor (MCHR, also designated SLC-1) is a glycosylated G protein-coupled receptor.
MCHR mediates the effects of MCH through Gai and/or Gaq signaling and is expressed in several regions of the brain, including the cerebral cortex, amygdala, thalamus and hypothalamus.
MCH and MCHR have also been implicated in stimulating leptin expression and secretion in adipocytes, suggesting that the melanin-concentrating hormone and its receptor may be potential targets for modulating obesity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recomended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PMCH. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human PMCH.
