Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules.
These enzymes are generally calcium-dependent and have been found both intra- and extracellularly.
By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids.
One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids, many of which are potent mediators of inflammation.
As a member of the PLA2 family, group IID sPLA2, also known as PLA2G2D (phosphatidylcholine 2-acylhydrolase 2D), sPLA2-IID or SPLASH, is a 145aa secreted protein that is broadly expressed but found at highest levels in pancreas and spleen.
Group IID sPLA2 catalyzes the reaction of phosphatidylcholine and water into 1-acylglycerophosphocholine and carboxylate, and has been liked to body weight loss in patients with chronic obstructive pulmonary disease (COPD), as well as lymphotoxin deficiency.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PLA2G2D.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PLA2G2D.
