The PIP5K family is divided into type I, type II and type III proteins, each of which phosphorylates distinct substrates.
The PIP5K proteins contain an activation loop which determines their enzymatic specificity and subcellular targeting.
Phosphatidylinositol-4- phosphate-5-kinase, also known as PIP5KIII or PIP5K3, synthesizes phosphatidylinositol-4,5- bisphosphate, which regulates various processes including cell proliferation, survival, membrane trafficking and cytoskeletal organization.
Located in the membrane of the endosome, PIP5KIII contains an FYVE-type zinc finger and catalyzes the rate-limiting step in the production of phosphatidylinositol, a phospholipid that plays a role in Actin dynamics.
Defects in the gene encoding PIP5KIII lead to corneal fleck dystrophy (CFD), an autosomal disorder in which small white flecks are found throughout the corneal stroma.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PIP5K3. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human PIP5K3.
