PHYHD1 (phytanoyl-CoA dioxygenase domain containing 1) is a 291aa protein belonging to the PHYH family and the PHYHD1 subfamily.
Encoded by a gene that maps to human chromosome 9q34.11, PHYHD1 exists as three alternatively spliced isoforms and likely functions as an a-ketoglutatare-dependent dioxygenase.
PHYHD1 participates in metal ion binding and oxidoreductase activity, thereby acting on single donors with incorporation of two atoms of oxygen.
Related to PHYH (phytanoyl-CoA 2-hydroxylase), an Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenase that catalyzes the initial a-oxidation step in the degradation of phytenic acid in peroxisomes, PHYHD1 similarly exhibits additional homologues in a wide range of metazoans and bacteria.
PHYHD1 also may play a role in DNA methylation in early postnatal liver development and mammalian differentiation.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PHYHD1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human PHYHD1.
