PHIP (pleckstrin homology domain interacting protein), also known as ndrp or WDR11, is a 1821aa protein that contains eight N-terminal WD40 repeats and two bromodomains.
It is expressed in skeletal muscle (localizing to the cytosol and nucleus) and primary B cells (localizing to the nucleus) and acts as a transcriptional activator.
PHIP is known to interact with various members of the insulin receptor substrate (IRS) family.
The IRS family of proteins mediate insulin receptor signaling and play an important role in insulin-producing B cell proliferation and survival.
PHIP specifically associates with the PH domain of IRS-1 and may function to link IRS-1 to insulin receptors, indicating a vital role of PHIP in the regulation of insulin signaling.
Applications:Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunocytochemistry/Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2,000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PHIP. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant full-length human PHIP protein.
