Gap junctions are formed by a hexameric group of proteins called connexins for the transport of low molecular weight proteins from cell to cell.
Connexins, which are present in all metazoan organisms, serve diverse functions ranging from control of cell growth and differentiation to electric conduction in excitable tissues.
Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication.
The pannexin gene family encodes a second class of putative gap junction proteins.
Pannexins are highly conserved in invertebrates and mammals, indicating the importance of their gap junctional coupling function.
Mammalian Pannexin-1 and Pannexin-3 are closely related, while Pannexin-2 is a more distant relation.
Pannexin-1 is a transmembrane protein that forms calcium-permeable gap junctions between adjacent cells and in the endoplasmic reticulum.
In erythrocytes, Pannexin-1 forms a mechanosensitive ATP-permeable channel in the nonjunctional plasma membrane.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PANX1.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PANX1.
