84-4394-82　［取扱停止］ORC3 (Origin Recognition Complex Subunit 3, Origin Recognition Complex Subunit Latheo, LATHEO, ORC3L) 100ul　360921

特徴

• The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation.
• Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins.
• ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors.
• Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis.
• ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin.
• ORC4 and ORC6 are also expressed constantly throughout the cell cycle.
• ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble.
• The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.