Occludin is an integral membrane protein closely associated with the tight junctions of epithelial and endothelial cells.
Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops.
The protein exists in a variety of phosphorylated forms.
Phosphorylation is involved in regulating both the localization and the function of occludin.
Expression of occludin is up-regulated by polyunsaturated fatty acids, increasing transendothelial cell resistance and reducing cellular permeability to large molecules.
The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly expressed at cell-cell contact sites.
Applications:Suitable for Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human Occludin. Species Crossreactivity: mouse and rat
Clone # USB：A23
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human Occludin.
