OAT (ornithine aminotransferase (mitochondrial), ornithine-oxo-acid aminotransferase) is a 439aa protein encoded by the human gene OAT.
OAT belongs to the class III pyridoxal-phosphate-dependent aminotransferase family and is usually found as a homotetramer in the mitochondrion matrix.
OAT catalyzes the major catalytic reaction for ornithine.
Ornithinemia, presumably due to deficiency of ornithine ketoacid aminotransferase (OAT) has been found in patients with gyrate atrophy of the choroid and retina.
The clinical history of gyrate atrophy is usually night blindness that begins in late childhood, accompanied by sharply demarcated circular areas of chorioretinal atrophy.
During the second and third decades the areas of atrophy enlarge.
The hepatic cleavage product, hepatic OAT, is formed by cleaving a 25aa transit peptide from the N-terminus of the OAT precursor.
The renal form is produced by cleaving a 35aa transit peptide from the N-terminal.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human OAT. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human OAT.
