nidulans) is a ubiquitously expressed protein that is conserved from fungus to human.
Highly expressed in proliferating cells, NUDC localizes to the cytoplasm and nucleus, contains a CS domain and participates in neurogenesis, mitosis, nuclear migration and cytokinesis.
At the onset of mitosis, NUDC is phosphorylated by Plk.
This modification of NUDC is required for proper mitotic spindle formation, chromosome separation during mitosis, cytokinesis and cell proliferation.
In neurons and fibroblasts, NUDC forms a complex with LIS1 that localizes to the microtubule network and microtubule-organizing center and facilitates nuclear movement and transport in migrating neurons.
In addition, the NUDC/LIS1 complex can associate with the minus-end directed Dynein/Dynactin motor complex and, together, these complexes cooperate in the regulation of cytokinesis.
NUDCD3 is a 361aa protein that contains one CS domain and is phosphorylated upon DNA damage by either ATR or ATM.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human NUDC. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human NUDC.
