CNS white matter is selectively inhibitory for axonal outgrowth.
Nogo is an oligodendrocyte- specific member of the reticulon family and is a component of CNS white matter that prevents axonal regeneration in the adult CNS.
Nogo is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum.
The extracellular domain of Nogo, designated Nogo-66 inhibits axonal extension, but does not alter non-neuronal cell morphology.
Expression of a brain-specific, leucine-rich-repeat protein with high affinity for Nogo-66, the Nogo-66 receptor (Nogo-R), is sufficient to impart Nogo-66 axonal inhibition to unresponsive neurons.
Disruption of the interaction between Nogo-66 and Nogo-R potentially provides for enhanced recovery after human CNS injury.
Nogo-R is widely expressed in the brain, with the highest levels of expression in the gray matter of the CNS.
In addition, low levels of Nogo-R mRNA are expressed in heart and kidney.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Nogo-R. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Nogo-R.
