Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes.
Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane.
In mammals, two Nidogen proteins, Nidogen and Nidogen-2, interact at comparable levels with Collagen I, IV and Perlecan, serving to stabilize basement membranes and playing a major role in embryogenesis.
The two isoforms have a similar shape, consisting of three globular domains, and co-localize in vessel walls and other basement membrane zones.
Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, and is widely distributed in basement membranes in heart, placenta, bone and, to a lesser extent, in pancreas, kidney and skeletal muscle.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human NID2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human NID2.
