NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1,2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation.
The NEIL proteins belong to the FPG family of proteins.
They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site.
NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2.
NEIL1 is a ubiquitiously expressed protein that is up-regulated during S-phase.
NEIL2 is expressed primarily in testis, heart, skeletal muscle, placenta, brain, kidney and liver while NEIL3 is detected primarily in thymus and testis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human NEIL2. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to residues in human NEIL2.
