Myosin, the major component of thick muscle filaments, is a long asymmetric molecule containing a globular head and a long tail.
Activation of smooth and cardiac/ventricular muscle primarily involves pathways which increase calcium and myosin phosphorylation, resulting in contraction.
Myosin in vertebrate striated muscle is composed of two heavy chains and four light chains.
There are two distinct types of light chains: the phosphorylatable, regulatory or MLC2 type; and the nonphosphorylatable, alkali or MLC1 and MLC3 types.
Myosin light chain phosphatase acts to regulate muscle contraction by de-phosphorylating activated myosin light chain.
The role of myosin alkali light chains in vertebrate skeletal muscle is poorly understood, although alkali light chains in smooth muscle may be involved with the active site of myosin.
Several isoforms of myosin alkali light chains have been identified, encoded by a family of myosin light chain genes.
Each is associated with different muscle types.
Human myosin light chain can be used as a cardiac marker.
Myosin light chain 3, encoded by MYL3, is an alkali light chain also referred to as both the ventricular isoform (MLC1v) and slow skeletal muscle isoform.
Myosin light chain 3 proteins in human and mouse share 91% sequence identity overall.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human MYL3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human MYL3.
