Matrix metalloproteinases (MMPs) are highly homologous Zn2+ endopeptidases involved in extracellular matrix (ECM) breakdown.
MMP-20 (enamelysin) is involved in the degradation of various components of the ECM during development, hemostasis and pathological conditions.
The domain organization of MMP-20 is similar to other MMPs, including a signal peptide, a prodomain with the conserved motif PRCGVPD involved in maintaining enzyme latency, a catalytic domain with a Zn-binding site, and a COOH-terminal fragment similar to the sequence of hemopexin.
MMP-20 is expressed during the early through middle stages of enamel development at which time it likely hydrolyzes Amelogenin, a major protein component of the enamel matrix.
The expression pattern of MMP-20 in the enamel organ indicates that it may be involved in the turnover of ECM proteins during tooth development and enamel formation.
Human MMP-20 maps to chromosome 11q22.3, clustered to at least seven other members of the MMP gene family.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human MMP20. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human MMP20.
