Human milk-fat globule (MFG) is abundant in human breast milk and is composed of secreted lipids encapsulated by plasma membranes from the epithelial cells of mammary glands.
MFG membranes are composed of various glycoproteins that serve as markers for differentiated carcinomas.
MFG-E8 (Milk fat globule-EGF factor 8), also known as Lactadherin or BA46, is a 387aa peripheral membrane protein that localizes to the membrane of a variety of tissues, including mammary epithelial surfaces, and contains one EGF-like domain and two F5/8 type C domains.
Functioning as a specific ligand for Integrin beta 5 and Integrin beta3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis.
Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection.
Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human MFGE8.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human MFGE8.
