In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II).
The mediator complex is one such multiprotein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription.
Med11 (mediator complex subunit 11) is a 117aa nuclear protein and component of the mediator complex.
The gene encoding Med11 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1200 genes.
Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1.
Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome.
Like p53, BRCA1 is directly involved in DNA repair, and is associated with predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human MED11. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human MED11.
