84-4391-99　［取扱停止］MBTPS2 (Membrane-bound Transcription Factor site-2 Protease (EC:3.4.24.85), Endopeptidase S2P, Sterol Regulatory Element-binding Proteins intramembrane Protease, SREBPs intramembrane Protease, MBTPS2, S2P) 100ul　360742

特徴

• The transcription factors SREBPs (sterol regulatory element binding proteins) span the ER membrane and, in response to sterol depletion, the N-terminal domain of SREBPs are proteolytically activated, released from the membrane and then translocate to the nucleus where they induce the expression of genes regulating cholesterol metabolism.
• This proteolytic activation requires the sequential cleavage of SREBPs at Site-1, within the lumen of the ER, followed by cleavage at Site-2, within the first transmembrane domain.
• The cleavage at Site-1 separates the N-terminal and C-terminal domains of the protein and it requires the serine protease, S1P (Site-1 protease).
• Site-2 is subsequently processed by a putative zinc metalloprotease S2P, which releases the activated N-terminal domain for nuclear translocation.
• This proteolytic pathway is tightly regulated by sterol levels and is under the control of SCAP (SREBP cleavage-activating protein).
• SCAP, a sterol sensor, is latently bound to the C-terminal regulatory domains of the SREBPs, and it regulates cleavage of SREBPs at Site-1.
• Sterol levels influence the activity of SCAP, as SCAP is activated only in sterol-depleted cells and it inhibited by sterol accumulation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.