All members of the Src gene family of tyrosine kinases are characterized by a C-terminal domain tyrosine, Y527 in the case of Src p60, which is highly phosphorylated in the inactive form of the enzyme, while phosphorylated to a much lesser extent when the enzyme is active.
For instance, a mutant of c-Src, in which Y527 is replaced by phenylalanine, is transforming and displays 5 to 10-fold elevated kinase activity compared to its normal counterpart.
Csk has been identified as a Src related tyrosine kinase having both SH2 and SH3 domains and a catalytic domain but lacking sequences N-terminal to the SH3 domain as well as the C-terminal regulatory sequences.
Csk phosphorylates Src on Y527 and also down regulates Fyn, Fyn and Lck by tyrosine phosphorylation of C-terminal regulatory sites.
A Csk-like protein-tyrosine kinase of mouse cell origin (Ctk), also designated Ntk, and its human homolog, Lsk, have also been described.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human MATK. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human MATK.
