Methionine adenosyltransferase (MAT) catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver.
Two different genes, MAT1A and MAT2A, encode a liver specific and non-liver specific form of MAT, designated MAT Ialpha and MAT IIalpha, respectively.
Inactivation of the liver specific gene product, designated MAT I/III, associates with liver diseases such as cirrhosis.
MAT Ialpha expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT IIalpha present a dedifferentiated phenotype and lowered AdoMet synthesis.
Likewise, NF-kB and TNFalpha cause a switch from MAT Ialpha to MAT IIalpha expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human MAT1A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human MAT1A.
