The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis.
The serine/threonine (Ser/Thr) protein kinases are a group of proteins that are intimately involved in this process.
MARK4 (MAP/microtubule affinity- regulating kinase 4), also known as MARKL1 or KIAA1860, is a 752aa protein that contains one UBA domain, one protein kinase domain and one kinase-associated domain and belongs to the Ser/Thr protein kinase family.
Expressed ubiquitously as two alternatively spliced isoforms, one of which is brain-specific, MARK4 uses ATP to catalyze the phosphorylation of target proteins and is thought to be involved in cell growth.
MARK4 is upregulated in hepatocellular carcinoma cells, suggesting a role for MARK4 in tumorigenesis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human MARK4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human MARK4.
