84-4391-80　［取扱停止］MAPRE2 (Microtubule-Associated Protein RP/EB Family Member 2, APC-binding Protein EB2, End-binding Protein 2, EB2, MAPRE2, RP1) 50ul　360733

特徴

• APC (adenomatous polyposis coli) is a tumor suppressor gene that is frequently mutated in colorectal cancers and is one of the earliest indicators of colorectal carcinogenesis.
• APC is widely expressed and is largely similar to the related brain-specific homolog APCL.
• These proteins both associate with beta-catenin and functionally regulate the levels of intracellular beta-catenin.
• Additionally, these homologs interact with the microtubule cytoskeletal protein EB1 to regulate cell cycle progression or growth control.
• EB1 family proteins (EB1, RP1 (EB2) and EB3) interact with cytoplasmic microtubules ininterphase cells, with mitotic spindles, and with the APC tumor suppressor gene.
• The functional inactivation of the APC gene product is a key event in colorectal tumorigenesis.
• RP1 is localized in the plus ends of microtubule networks in the presence or absence of APC.
• The gene which encodes RP1 maps to human chromosome 18q12.1.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.