APC (adenomatous polyposis coli) is a tumor suppressor gene that is frequently mutated in colorectal cancers and is one of the earliest indicators of colorectal carcinogenesis.
APC is widely expressed and is largely similar to the related brain-specific homolog APCL.
These proteins both associate with B-catenin and functionally regulate the levels of intracellular B-catenin.
Additionally, these homologs interact with the microtubule cytoskeletal protein EB1 to regulate cell cycle progression or growth control.
EB1 family proteins (EB1, RP1 (EB2) and EB3) interact with cytoplasmic microtubules ininterphase cells, with mitotic spindles, and with the APC tumor suppressor gene.
The functional inactivation of the APC gene product is a key event in colorectal tumorigenesis.
RP1 is localized in the plus ends of microtubule networks in the presence or absence of APC.
The gene which encodes RP1 maps to human chromosome 18q12.1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human MAPRE2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human MAPRE2.
