Lipopolysaccharide has been shown to induce tyrosine phosphorylation of a unique protein, which is designated p38.
p38 is a member of the MAP kinase family with features most closely resembling those of the Saccharomyces cerevisiae protein Hog1.
p38 and Hog1 share a TGY phosphorylation sequence, whereas most other MAP kinase family proteins have a TEY sequence.
A related protein, p38b, has been shown to phosphorylate ATF-2 at a 20-fold higher rate than p38, suggesting distinct substrate preferences.
Stress activated protein kinase-4, or SAPK4, also designated p38d, is a related protein that is phosphorylated by MKK6 in response to cytokines and cellular stresses.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human MAPK13. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human MAPK13.
