MAP1LC3B, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing.
Two forms of LC3 beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally.
LC3-I is formed by the removal of the C-terminal 22aa from newly synthesized LC3 beta, followed by the conversion of a fraction of LC3-I into LC3-II.
LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA.
This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation.
MAP LC3 may also be involved in formation of autophagosomal vacuoles.
It is expressed primarily in heart, testis, brain and skeletal muscle.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:1000Immunohistochemistry: 1:50- 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human MAP1LC3B. Species Crossreactivity: mouse and rat
Clone # USB：D23
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human MAP1LC3B.
