The LIM-only (LMO) proteins are nuclear factors characterized by a conserved LIM domain.
The LIM domain contains a cysteine-rich zinc-binding motif, present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system.
The deduced LMO7 protein is comprised of 1349aa residues, contains a characteristic zinc finger domain and a 3-prime UTR which possesses a short interspersed nucleotide element (SINE).
RT-PCR detects predominant expression of LMO7 in heart, lung, skeletal muscle, and kidney, moderate expression in liver, ovary, brain, pancreas, and testis, and little to no expression in spleen.
Research indicates that LMO7 is an afadin- and alpha-actinin-binding protein that connects the nectin-afadin and E-cadherin-catenin systems through alpha-actinin.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human LMO7. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human LMO7.
