During development, genetically distinct subtypes of motor neurons express unique combinations of LIM-type homeodomain factors, which regulate cell migration and guide motor axons to establish the fidelity of a binary choice in axonal trajectory.
The LIM gene family encodes a set of gene products, which carry the LIM domain, a unique cysteine-rich zinc-binding domain.
At least 40 members of this family have been identified in vertebrates and invertebrates, and are distributed into 4 groups according to the number of LIM domains and to the presence of homeodomains and kinase domains.
The overlapping expression of LHX1, LHX3, LHX4, Isl-1 and Isl-2 in developing motorneurons along the spinal column may influence the establishment of specific motorneuron subtypes.
The human LHX1 gene maps to chromosome 11p13-p12 and encodes a 384aa protein.
The human LHX1 transcript is assembled from five exons, which are separated by introns ranging in size from 93nt to 2.3kb.
The two LIM domains are entirely contained in the first and second exons, respectively, while the homeodomain is split into exons three and four.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.8, 0.05% sodium azide.
Specificity：Recognizes human LHX1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human LHX1.
