Cytokeratins are a subfamily of intermediate filament keratins that are characterized by a remarkable biochemical diversity, which is represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in isoelectric range between 4.9 and 7.8.
Cytokeratin 1 has the highest molecular weight, while Cytokeratin 19 has the lowest molecular weight.
The cytokeratins are divided into the type I and type II subgroups.
Type II family members comprise the basic to neutral members, Cytokeratins 1-8, while the type I group comprises the acidic members, Cytokeratins 9-20.
Various epithelia in the human body usually express cytokeratins which are characteristic of the type of epithelium and related to the degree of maturation or differentiation within said epithelium.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Cytokeratin 4 is expressed in differentiated layers of the mucosal and esophageal epithelia along with Cytokeratin 13.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human KRT4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human KRT4.
