The inter-a-trypsin inhibitor (ITI) family is a group of structurally related plasma serine protease inhibitors synthesized in the liver and built up from different combinations of three highly homologous heavy chains (ITI-HI, ITI-H2 and ITI-H3) and one light chain (Bikunin).
Another member of the ITI family, ITI-H4, harbors a proline-rich region (PRR) in its C-terminus.
ITI is a glycoprotein composed of three polypeptides linked by chondroitin sulphate: two heavy chains, ITI-H1 and ITI-H2, and Bikunin.
Bikunin confers the protease-inhibitor function of ITI.
The heavy chains of the ITI family, designated as SHAPs (for serum-derived hyaluronan-associated proteins), bind covalently to hyaluronic acid (HA), resulting in pericellular matrix stabilization.
While the ITI family is primarily composed of multi-polypeptide molecules, ITI-H4 is a single chain molecule.
Unlike other ITI family members, the gene transcriptions and products for rat and human ITI-H4 demonstrate marked differences, suggesting possible species-specific functions for ITI-H4.
The gene encoding human ITI-H4 maps to chromosome 3p21.1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human ITIH4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human ITIH4.
