The inter-alpha trypsin inhibitor (ITI) family is a group of structurally related plasma serine protease inhibitors synthesized in the liver and built up from different combinations of three highly homologous heavy chains (ITI-H1, ITI-H2 and ITI-H3) and one light chain (Bikunin).
A fourth member of the ITI family, ITI-H4 (also known as I a IH4P) harbors a Pro-rich region (PRR) in its C-terminal.
ITI is a glycoprotein composed of three polypeptides linked by chondroitin sulphate: two heavy chains, ITI-H1 and ITI-H2, and Bikunin.
Bikunin confers the protease- inhibitor function of ITI.
The heavy chains of the ITI family, designated as SHAPs (for serum-derived hyaluronan-associated proteins),bind covalently to hyaluronic acid (HA), resulting in pericellular matrix stabilization.
Although ITI-H1, ITI-H3 and Bikunin have antitumoral and antimetastatic properties in the cell, these proteins are also associated with malignant transformation of lung tissue.
ITI-H3 and Bikunin associate to form pre-a-trypsin inhibitor (PaI), a serine-proteinase inhibitor found in human serum.ITI-H3 mRNA levels increase in response to IL-6.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human ITIH3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues in human ITIH3.
