The production, survival and function of monocytes and macrophages are regulated by the macrophage colony-stimulating factor M-CSF through its tyrosine kinase receptor Fms.
Binding of M-CSF to Fms induces the tyrosine phosphorylation and association of SH2-containing inositol phosphatase SHIP with the phosphotyrosine-binding domain of Shc.
The SHIP protein hydrolyzes PtdIns P3 to PtdIns Ps and results in strong inhibition of cell growth.
SHIP is also a target for CD28, suggesting that SHIP may be involved in the regulation of T cell activation.
SHIP has several splice variants and is expressed during hematopoiesis and spermatogenesis.
SHIP-2, a homolog of SHIP, is expressed in both hemopoietic and non-hemopoietic cells.
In addition to T and B cells, spleen, thymus and lung are shown to coexpress SHIP and SHIP-2.
SHIP is also expressed in fibroblasts, heart, skeletal muscle and different brain areas and its expression is enhanced in TSH and EGF-stimulated cells.
Like SHIP, SHIP-2 is tyrosine-phosphorylated and associates with Shc after ligation of the B-cell receptor to FcgRII.
SHIP-2 causes cell cycle arrest in G1 phase in glioblastoma cells and plays a negative role in regulating the PI 3-kinase-PI 3-kinase B pathway.
Both SHIP and SHIP-2 mediate FcgRIIB signaling, including inhibition of proliferation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human INNPL1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human INNPL1.
