Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells.
IL-3 exerts its biological effects through a receptor which consists of a ligand-specific a subunit (IL-3Ra) and a signal transducing B subunit (IL-3RB) common to the IL-3/IL-5/GM-CSF receptors.
The a subunits are low-affinity ligand-binding proteins while the B subunits do not themselves bind ligand, but are required for high affinity binding by the a subunits.
The mouse IL-3 receptor has two distinct B subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF.
The murine B subunits are 91% homologous at the amino acid level but only 56% homologous to the human B subunit.
The carboxy-terminus of the B subunit has been shown to be necessary for activation of the MAP kinase signaling pathway.
Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human IL3RA. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in Human IL3RA.
