The IL-2 receptor is a multi component complex consisting of three subunits alpha, beta and gamma each of which is required for high-affinity binding of IL-2.
The a chain functions primarily in binding IL-2, where as the B and g chainscontribute to IL-2 binding and are essential to IL-2-induced activation of signaling pathways leading to T cell growth.
Both IL-4R and IL-7R were initially described as single chain, high-affinity ligand-binding cytokine receptors.
However, it is now well established that the IL-2Rg chain functions as a second subunit of the high affinity IL-4R and IL-7R receptors.
Consequently, the originally described subunits of these latter receptors are now referred to as IL-4Ra and IL-7Ra, respectively, while the common subunit isreferred to as gc.
Although the common g chain enhances ligand binding in these three cytokine receptors, it has no capacity to bind these ligands on its own.
There is evidence that the gc chain is also a subunit of IL-13R.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human IL2RA. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human IL2RA.
