IL-18 (also referred to as IL-1gamma) has been shown to augment the secretion of IFN-gamma from T lymphocytes and to increase NK cell activity in spleen cells.
IL-18 exhibits 19% and 12% identity with IL-1alpha and IL-beta, respectively, over the twelve beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family.
The unusual leader sequence of IL-18 may be analogous to the IL-1beta pro-domain, which must be cleaved by the serine protease ICE for optimal secretion and biological activity.
IL-18, which was originally described as IGIF (IFN-gamma-inducing factor), IL-18 is induced in mouse liver subsequent to challenge with lipopolysaccharide (LPS).
IL-18 binding protein (IL-18BP) functions as an inhibitor of the early Th1 response by binding to IL-18 and inhibiting IFN-gamma production.
IL-18BP is a member of the immunoglobulin superfamily and shares some homology to IL-1RII.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human IL18BP.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human IL18BP.
