The Th2 cytokine Interleukin-13 (IL-13) plays a critical role in allergen-induced airway hyper-responsiveness (AHR).
Two different receptors exist for IL-13, designated IL-13Ralpha1 and 2.
IL-13Ralpha1 exists as a heterodimer of IL-13Ralpha1 and IL-4Ralpha as a signaling subunit, whereas IL-13Ralpha2 acts as a decoy receptor for IL-13.
Furthermore, TNFalpha or IL-4 stimulation induces IL-13Ralpha2 upregulation, while IL-13Ralpha1 is constitutively expressed.
Cell surface localization of IL-13Ralpha2 abrogates IL-13 signaling, thus IL-13 induced translocation of the receptor from the cytoplasm provides a mechanism for negative-feedback of IL-13 signaling.
IL-13Ralpha1 expression is predominant in B cells, monocytes and T cells, whereas IL-13Ralpha2 expression is highest in glioma cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human IL13RA2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues aa251-296 in human IL13RA2.
