The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate N-terminal and C-terminal peptides.
Both proteins are secreted and appear to function in embryonic and imaginal disc patterning.
Several vertebrate homologs of Drosophila hh have been identified.
These include Sonic hedgehog (Shh), alternatively designated Vhh-1, Desert hedgehog (Dhh) and Indian hedgehog (Ihh).
Each contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions.
Shh resembles Drosophila hh in that it is processed to generate an N-terminal secreted peptide that is retained at or near the cell surface and a C-terminal glycosylated more diffusible peptide.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human IHH. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human IHH.
